基于RNAi技术沉默暗黑鳃金龟神经肽F基因的转录组分析OA

Neuropeptide F(NPF),a type of neuropeptide specific to invertebrates,plays a crucial role in insect growth,development,physiological activities,and behavior.However,its biological function in Holotrichia parallela,a major subterranean pest of peanut,remains unclear.In this study,RNA interference(RNAi)was used to silence the NPF gene in adult H.parallela,and comparative transcriptomic analysis was performed to investigate the gene expression changes in the head and antennal tissues.The results showed that NPF expression was significantly suppressed after 72 h post-NPF interfer-ence,achieving a silencing efficiency of 73.24％.Based on the transcriptome data,a total of 677 and 805 differentially expressed genes(DEGs)were identified in the head and antenna,respectively.GO and KEGG enrichment analyses revealed that these DEGs were significantly enriched in pathways related to immune response,metabolic processes,and other biological functions.Specifically,NPF knockdown led to the downregulation of several key genes in the Toll and Imd signaling pathway,suggesting a potential role for NPF as a positive regulator of innate immunity in insects.Meanwhile,significant changes were observed in the expression of genes related to carbohydrate and lipid metabolism,supporting the involvement of NPF in energy homeostasis.In ad-dition,differential expression was detected in the antennal genes mapping to the sensory system pathway,implying that NPF may al-so plays a role in the regulation of chemosensation and olfaction.In conclusion,the multiple roles of NPF in the physiological regula-tion of H.parallela from a transcriptomic perspective was elucidated in this study,providing a theoretical basis for exploring the regulatory mechanisms of this neuropeptide in coleopteran pests and for developing sustainable pest control strategies.