花生半胱氨酸氧化酶基因AhPCO的克隆与表达分析OA

Research on plant oxygen-sensing mechanisms has demonstrated that cysteine oxidase genes(PCOs)serve as critical oxygen sensors,modulating oxygen-responsive signaling pathways to support the normal growth and development of plant under stress conditions,thereby conferring a significant survival advantage.The coding sequence(CDS)of the peanut AhPCO gene was successfully cloned in this study,which spans 843 bp and encodes a protein consisting of 281 amino acids with a theoret-ical isoelectric point(pI)of 6.06.The protein exhibits hydrophilicity,instability,and lack of signal peptide,and is primarily lo-calized in the nucleus and cytoplasm.Phylogenetic analysis revealed a close evolutionary relationship between the AhPCO protein and AtPCO1 from Arabidopsis thaliana.Analysis of the AhPCO promoter region suggested that the transcription may be in-duced by abscisic acid(ABA)and anaerobic conditions.The qPCR results showed that AhPCO exhibits tissue-specific expres-sion,with the expression levels in different peanut tissues as the following order:seed＞stem＞root＞leaf＞flower.Stress treatment experiments showed that,compared with the control group,AhPCO expression was significantly upregulated after 2 h of high-temperature treatment and 12 h of salt stress.In contrast,AhPCO expression decreased significantly after 15 d of drought stress,and increased sharply while following 1 d of rehydration.Although the expression gradually declined over the subsequent 2 to 4 d,it remained higher than that in the control.The AhPCO gene is significantly regulated by various abiotic stresses,including high temperature,salinity,drought,and rehydration,and exhibits tissue-specific expression patterns.It may play a crucial regulatory role in the stress response and recovery processes of peanuts under environmental stress condi-tions.This study lays a theoretical foundation for elucidating the function of AhPCO gene and provides potential genetic resources for breeding stress-tolerant,high-quality peanut varieties.