丹参PERK基因家族的鉴定及表达分析OA

Proline-rich extensin-like receptor kinase(PERKs)play critical roles in plant growth,development,and stress responses.To elucidate the functions of the PERK gene family in medicinal plant Salvia miltiorrhiza,we performed a comprehensive genome-wide identification and analysis,such as chromosomal distribution,collinear relationships,phylogenetic relationships,conserved motifs,and cis-acting elements.Furthermore,expression patterns of SmPERK genes across different tissues and under salicylic acid(SA),abscisic acid(ABA),and cucumber mosaic virus(CMV)stress were investigated using transcriptome sequencing and quantitative real-time PCR(qRT-PCR).We identified 20 PERK genes,which were unevenly distributed across six chromosomes.Synteny analysis suggested that gene duplication events were the primary drivers of SmPERK family expansion.Expression profiling revealed distinct tissue-specific patterns:SmPERK12 was highly expressed in flowers,whereas SmPERK11 and SmPERK14 showed preferential expression in roots and stems.Several SmPERK genes responded to SA and ABA treatments,with SmPERK14 exhibiting particularly strong induction by ABA.Upon CMV infection,the expression of most family members was altered,with SmPERK13 and SmPERK14 being significantly upregulated.This study systematically characterizes the SmPERK gene family,clarifying its potential roles in tissue differentiation,hormone signaling,and antiviral defense.The identification of key candidate genes,particularly SmPERK13 and SmPERK14,provides a significant theoretical basis and valuable genetic resources for further understanding the regulatory network governing growth,development,and environmental adaptation mediated by the PERK gene family in S.miltiorrhiza.