魔芋苯丙氨酸解氨酶基因家族鉴定及表达分析OA

Phenylalanine ammonia lyase(PAL)is indispensable in plants as the primary rate-limiting enzyme of phenylpropanoid metabolism,which underpins both growth and secondary metabolic pathways.To investigate the functions of PAL gene in the growth and development of Amorphophallus konjac,we conducted comprehensive analyses of AkPAL family members,including physicochemical properties,phylogenetic tree,conserved motifs/domains,and promoter characteristics.Additionally,expression levels of AkPALs across various tissues and during corm growth stages of A.konjac were investigated.Quantitative real-time PCR(qRT-PCR)was utilized to assess the expression of AkPALs following lanthanum induction.The results identified 7 AkPAL genes distributed across 5 chromosomes,encoding proteins of 212-759 amino acids that clustered into three phylogenetic clades with closer relationships to monocots.The protein secondary structure composition was predominantly characterized by α-helical segments and random coil conformations.Promoter regions exhibited significant enrichment of light,hormone,and stress-responsive cis-elements.Moreover,integrated transcriptomic and qRT-PCR analyses showed that AkPAL4/5 play significant roles in regulating both corm development and alkaloid biosynthesis in A.konjac.These results provide a theoretical reference for further studies on AkPALs genes and their biological functions in A.konjac.