不同养殖模式和温度对雌性克氏原螯虾性腺发育的影响OA

In order to elucidate the differences in gonadal development of Procambarus clarkii under different culture modes and to investigate the effect of temperature as a key environmental variable,the study measured and observed the ovarian development of female Procambarus clarkii cultured in rice fields,ponds,and indoor cement ponds in Jiading area,Shanghai,in the middle of each month from May to September 2024,and set up three temperature-controlled groups,including 22℃,26℃,and 30℃,and one natural environmental temperature culture group for 28 d indoor culture experiments to deeply analyze the characteristics of gonadal development under different temperatures.The results showed that there was no significant difference in the progress of gonadal development between rice field and pond culture(P>0.05),and the gonadal development of P.clarkii in indoor cement pond culture was faster than that in rice field and pond culture,and in the middle of September,85％of female ovaries matured to stage Ⅴ,and 15％of female shrimp had finished spawning,which was 1 month earlier than that in rice field and pond culture.The indoor temperature control experiments found that the gonadosomatic indexes of three temperature control groups were significantly higher than those of the natural environment temperature culture group(P<0.05),indicating that the temperature difference between day and night significantly affected the gonadal development of P.clarkii,among which the 26℃group had the highest gonadosomatic indexes,the fastest development of gonad,and the percentage of stage Ⅳ ovary was 63.33％,which was better than that of the other groups.In conclusion,appropriate temperature regulation has a significant effect on the gonadal development of P.clarkii,26℃is a more suitable temperature,which is conducive to the early reproduction of P.clarkii.This study provides a reference for assessing the suitability of Procambarus clarkii aquaculture.