基于网络药理学方法探讨活血药治疗IgA肾病的作用机制OA

Objective:To discuss the mechanism of blood-activating drugs in the treatment of IgA nephropathy based on network pharmacology.Methods:TCMSP was utilized to screen and obtain the active ingredients of blood-activating drugs and its targets of action.IgA nephropathy related targets were searched and screened from Online Mendelian Inheritance in MaN(OMIM)and the GeneCards database of human genes,Venn diagram was used to obtain the shared targets between medicine and disease.PPI network was constructed using STRING platform,GO and KEGG functional enrichment analysis of the network were conducted using Metascape platform.Cytoscape was adopted to build medicine-active ingredients-targets-pathway network.Results:The study has identified 85 active ingredients from the drugs,predicted 239 potential targets of the drugs and 667 IgA nephropathy-related targets.Among which,there were 82 targets of active ingredients from blood-activating drugs involved in IgA nephropathy.PPI network model covered 82 nodes and 312 edges,and the key targets contained signal transduction and transcription activator 3,TNF,IL-6,IL-10,mitogen-activated protein kinase 1,protein kinase B antibody and mitogen-activated protein kinase 14.GO enrichment analysis involved growth factor receptor binding pathway,cytokine receptor binding pathway,DNA-transcription factor binding pathway.KEGG enrichment analysis displayed that the key signaling pathways were AGE-RAGE signaling pathway,TNF signaling pathway,MAPK signaling pathway and interleukin 17 signaling pathway of diabetic complications.Conclusion:Blood-activating drugs could exert synergistic effects on IgA nephropathy possibly through multi-ingredient,multi-target and multi-pathway.