从内源性抗菌肽研究尿感方对膀胱上皮细胞感染早期防御响应的影响OA

Objective:To observe the effects of urinary-infection prescription on early defensive response in bladder epithelial cells(BECs)infection via the expressions of AMPs,and to survey its mechanism from IL-6/Stat3 signaling pathway.Methods:RT-PCR was applied to detect the expressions of AMPs including CAMP,DEFB1,LCN2,RNases4 and RNase7 mRNA in BECs at early stage of uropathogenic escherichia coli(UPEC)infection,to observe bacterial colonization rate,meanwhile ELISA was used to measure the levels of IL-6,Western blotting to monitor the expressions of p-Stat3 protein.Results:No significant difference existed in the expressions of mRNA of AMPs including CAMP,DEFB1,LCN2,RNases4 and Nases7,bacterial colonization rate,the levels of IL-6 and p-Stat3 when 5%and 10%normal rat urine control groups were compared with the model group(P>0.05);the upregulation of Nases7 mRNA was observed when 5%drug-containing rat urine group was compared with 5%normal rat urine control group(P<0.05);10%drug-containing rat urine group demonstrated the increase in mRNA expressions of AMPs including CAMP,DEFB1,LCN2,RNases4 and Nases7,the levels of IL-6 and p-Stat3,while the decrease in bacterial colonization rate when compared with 10%normal rat urine control group(P<0.05).Conclusion:Urinary-infection prescription could enhance the expressions of AMPs in BECs during the early stage of infection,enhance rapid defense response of BECs against pathogenic bacteria,and reduce bacterial colonization rate,and part of the mechanism is related to IL-6/Stat3 signaling pathway.