固相支撑液液萃取/液相色谱-串联质谱法检测人血清中10种抗精神药物OA

This study developed a rapid and high-throughput liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for the simultaneous quantification of 10 antipsychotic drugs in hu-man serum.The sample preparation process employed solid-phase supported liquid-liquid extraction(SLE),which offers improved reproducibility and reduced matrix effects compared to traditional liq-uid-liquid extraction.Methyl tert-butyl ether(MTBE)was used as the elution solvent due to its high extraction efficiency and low residue.After extraction,the eluate was concentrated under a gentle ni-trogen blow-down process to ensure minimal loss of volatile analytes and then reconstituted with a 50％methanol-water solution to enhance compatibility with the LC mobile phase.The chromatographic conditions were meticulously optimized to enhance sensitivity and specificity for the target analytes.The method demonstrated excellent linearity over the tested concentration ranges,with correlation co-efficients(r)greater than 0.999 for all 10 antipsychotics.The limits of detection(LOD)and quantifi-cation(LOQ)were in the ranges of 0.002-0.075 ng/mL and 0.005-0.250 ng/mL,respectively,in-dicating high sensitivity capable of detecting trace levels in clinical samples.The recoveries ranged from 87.3％to 112％,confirming the efficiency and consistency of the extraction process.Both intra-day and inter-day precision,expressed as relative standard deviations(RSDs),were not more than 13％,highlighting the method's reproducibility under varied conditions.Matrix effects were evaluat-ed and found to be between 87.2％and 115％with RSD values less than 15％,demonstrating minimal impact from serum components on the accuracy of the measurements.Furthermore,the method was fully validated according to international guidelines and proven to be robust and reliable for high-throughput applications.This method offers a practical,sensitive,and reproducible approach for the simultaneous determination of multiple antipsychotic drugs in serum,supporting therapeutic drug monitoring(TDM)and clinical research in psychiatric treatment.Its high throughput capability,min-imal matrix interference,and reliability at low concentrations make it an attractive and modern alter-native to existing analytical methods.