基于网络药理学和HPLC谱效关系的黄连改善2型糖尿病潜在质量标志物研究OA

An integrated strategy combining network pharmacology with high performance liquid chromatography(HPLC)fingerprint spectrum-effect correlation was developed to identify the key quality markers of Coptidis Rhizoma for type 2 diabetes mellitus.A compound-target-disease net-work was constructed,prioritizing six bioactive alkaloids—coptisine,jatrorrhizine,epiberberine,palmatine,obacunone and berberine—based on topological centrality.HPLC fingerprints of ten batches were established,and in vitro α-glucosidase inhibition and antioxidant assays were conduct-ed.Grey relational and bivariate correlation analyses demonstrated significant positive associations between the hypoglycemic effect and the levels of berberine,obacunone,epiberberine and copti-sine.Principal component analysis and hierarchical clustering revealed marked inter-batch composi-tional divergence.An activity-oriented quality evaluation paradigm was thus established,transcend-ing traditional compound-centric approaches and definitively designating the four aforementioned con-stituents as core quality markers for Coptidis Rhizoma in type 2 diabetes mellitus management.These findings provide a robust scientific foundation for the standardization,quality control and precision clinical application of this botanical drug,advancing the modernization of traditional Chinese medi-cine and evidence-based therapeutics.