中国食品动物源1,4,[5],12:i:-沙门氏菌致病性岛、运动性、生物膜形成和大蜡冥致死特征研究OA
Pathogenic Island Genes,Motility,Biofilm Formation and Galleria mellonella Mortality Characteristics of 1,4,[5],12:i:-Salmonella from Chinese Food Animal Sources
为了研究国内食品动物源单相变异鼠伤寒沙门氏菌(1,4,[5],12:i:-沙门氏菌)的毒力因子、运动性、生物膜形成能力及其对大蜡螟的致死特征.采用全基因组测序分析致病岛基因携带情况;通过结晶紫染色法评估生物膜形成能力;利用半固体琼脂泳动试验检测菌株运动能力;通过大蜡螟幼虫体内感染试验评价菌株毒力.结果:59株1,4,[5],12:i:-沙门氏菌100％携带致病岛基因,最高可达7种不同的致病岛毒力基因类型.32.20％的菌株具有中等或较强生物膜形成能力;部分菌株表现出较强泳动能力,最大泳动直径为0.93 cm.16株受试菌对大蜡螟均表现出较强致病性,感染6 d后致死率达100％,其中菌株94在感染第1天即引起全部死亡.结论:中国食品动物源1,4,[5],12:i:-沙门氏菌在基因水平、生物膜形成、运动能力及体内毒力方面表现出复杂多样的特征,其毒力可能受多因素共同影响.研究结果为该血清型沙门氏菌的监测与防控提供了基础数据.
To study the pathogenic island genes,motility,biofilm formation and Galleria mellonella mortality charac-teristics of monophasic variant of S.Typhimurium 4,[5],12:i:-(MVST)in food animals in China.The virulence char-acteristics of MVST were investigated by whole genome sequencing analysis,crystal violet staining to assess biofilm formation,swimming motility test and Galleria mellonella in vivo infection.The results showed that 59 MVST carried 100％Salmonella pathogenic island(SPI)genes,and up to 7 different SPI types.MVST can form biofilm in vitro,and 32.20％have moderate or strong biofilm formation ability.Several MVST had strong swimming ability,with a maxi-mum swimming diameter of 0.93 cm.Most of the 16 isolates presented highly virulent profiles,which led to high mor-tality rates and blackening of Galleria mellonella.In this study,the characteristics of MVST from Chinese food animal sources were investigated from the aspects of gene level,biofilm formation,mobility and mortality rates.The results suggested that the characteristics of MVST were relatively complex,which might be influenced by various different factors.This will provide a basis for surveillance of Salmonella isolates.
沈焕钧;王心远;黄恺祁;许成钢;宋厚辉;蒋红霞;赵秋云
浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300华南农业大学兽医学院,广东 广州 510642浙江农林大学动物科技学院,动物医学院,浙江 杭州 311300
农业科技
1,4,[5],12:i:-沙门氏菌致病性岛生物膜泳动能力致死率毒力特征
1,4,[5],12:i:-SalmonellaPathogenic islandBiofilmSwimming abilityMortality rateVirulence characteristics
《动物医学进展》 2026 (1)
40-46,7
浙江省自然科学基金重点项目(Z24C010001):浙江农林大学科研发展基金人才启动基金资助项目(203402009901)浙江农林大学大学生科研训练项目(2024kx0081)
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