云南部分地区羊芽囊原虫感染情况调查及亚型鉴定OA

To investigate the infection and subtype distribution of Blastocystis sp.in sheep and goats in Yun-nan province,fecal samples were collected from sheep and goats in Kunming city,Honghe prefecture,and Chuxiong prefecture from July 2023 to January 2024.Genomic DNA was extracted from the fecal samples,and PCR amplification was performed based on the small subunit ribosomal RNA(SSU rRNA)gene of Blastocystis sp.After sequencing the positive samples,Blast alignment was performed on NCBI to identify the species and gene subtypes.Phylogenetic tree was constructed using neighbor-joining method(NJ)of MEGA 11.0 software.The factors affecting the detection rate of Blastocystis sp.from sheep and goats were analyzed by x2 test using SPSS 20.0 software.A total 138 of sheep and goats fecal samples were collected,and 35(25.36％)fecal samples were amplified with SSU rRNA gene fragments of Blastocystis sp.The de-tection rates in Kunming city,Chuxiong prefecture and Honghe prefecture were 30.00％,24.07％and 16.67％,respectively.The detection rates of dairy goats,black goats and sheep were 30.00％,24.14％and 15.00％,respectively.There was no significant difference in the detection rate among different regions,vari-eties and breeding patterns.Blastocystis sp.were determined as ST1,ST10 and ST14 subtypes based on se-quence comparison.Three positive samples were identified as ST1 subtype,nine positive samples were iden-tified as ST14 subtype,while 23 positive samples were identified as ST10 subtype.The subtype ST10 was considered as the predominant subtype(23/35).Phylogenetic tree analysis showed that samples of ST1,ST10 and ST14 subtypes were all gathered together with the branches of their reference sequences,respec-tively.The topological structure of phylogentic analysis is in accordance with the previous studies.Blasto-cystis infection in sheep and goats is prevalent in three regions of Yunnan province,the predominant gene subtype is ST10.The results of present study show potential possibility of zoonosis of Blastocystis origina-ted from the zoonotic gene subtype ST1,which provides data support for further public health detection and prevention and control of Blastocysticosis.