尿液热带念珠菌qPCR快速检测方法的建立与临床应用价值OA
Establishment and clinical application of quantitative PCR rapid detection method for urine Candida tropicalis
目的 建立尿液热带念珠菌的qPCR快速检测方法,并评估其临床应用价值.方法 在热带念珠菌核糖体RNA编码基因(rDNA)之间内转录间隔区1(ITS1)上设计特异性引物,通过qPCR分析体系建立尿液热带念珠菌的检测方法;从检出限、定量低限和特异度等方面评价该方法,并通过尿液标本检测评估其临床应用.结果 该检测方法对纯菌液的检出限和定量低限为102 CFU/mL,对尿液标本的检出限和定量低限为103 CFU/mL,对含有热带念珠菌的尿液标本均有扩增,对培养阴性或含有其他病原菌的尿液标本均无扩增.结论 本研究建立的尿液热带念珠菌qPCR快速检测方法定量低限低、特异度高.
Objective To establish a quantitative PCR(qPCR)rapid detection method for urine Candi-da tropicalis,and to evaluate its clinical application value.Methods The specific primers were designed on the intra-transcriptional spacer 1(ITS1)between the ribosomal RNA-coding genes(rDNA)of Candida tropicalis.The detection method for Candida tropicalis was constructed by the qPCR analysis system;this method was e-valuated by the limit of detection(LoD),limit of quantitation(LloQ)and analytical specificity.Its clinical ap-plication was evaluated by the urine sample detection.Results The LoD and LloQ of the method for pure bac-terial liquid were 102 CFU/mL.The LoD and LloQ of the method for urine samples were 103 CFU/mL.All u-rine specimens containing Candida tropicalis had amplification and had no amplification for the urine samples with negative culture or containing other pathogenic bacteria.Conclusion This study has successfully con-structed the qPCR rapid detection method for urine Candida tropicalis with low LloQ and high specificity.
沈菁;李孝辉;孙静芳;陈燕;徐银海;郭毅
徐州医科大学附属医院检验科,江苏 徐州 221002徐州矿务集团总医院检验科,江苏 徐州 221006徐州医科大学附属医院检验科,江苏 徐州 221002徐州医科大学医学技术学院,江苏 徐州 221004徐州医科大学附属医院检验科,江苏 徐州 221002徐州矿务集团总医院检验科,江苏 徐州 221006
医药卫生
念珠菌尿热带念珠菌实时荧光定量PCR内转录间隔序列
candiduriaCandida tropicalisreal-time fluorescence quantitative PCRinternal transcrip-tional spacer sequence
《重庆医学》 2026 (1)
57-61,5
国家自然科学基金青年科学基金项目(82402722)徐州市科技项目2023年度医药卫生面上项目(KC23265).
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