新疆高寒草地3种植物根际土壤真菌群落结构及功能多样性OA

This research investigated the rhizosphere fungal community structure and ecological functions among alpine grassland plant species(Taraxacum mongolicum,Poa annua and Daucus carota)in the alpine grassland of Kizilsu Kirgiz Autonomous Prefecture,Xinjiang,using high-throughput sequencing technology.Correlations between soil physicochemical properties and fungal community structure were also analyzed.No significant differences in fungal alpha diversity were found among plant species.However,an analysis of similarities(ANOSIM)test indicated that the fungal community structure of P.annua was significantly different from that of the other two plant species.The dominant fungal phyla were identified as Ascomycota,Basidiomycota,and Mortierellomycota,while the predominant genera included Archaeorhizomyces,Hygrocybe,Thelebolus,and Podospora.LefSe analysis identified Podospora,Hygrocybe,and Archaeorhizomyces as biomarkers significantly associated with T.mongolicum,P.annua,and D.carota,respectively.FUNGuild functional annotation revealed that nutritional modes of fungi across all samples were predominantly saprotrophic(27.1%-28.6%),with undefined saprotroph fungi being the most dominant functional group(18.5%-24.9%).Soil organic matter,alkali-hydrolyzable nitrogen,available phosphorus,and total nitrogen were identified as key determinants influencing fungal diversity.In conclusion,the structure and composition of rhizosphere fungal communities in the alpine grassland of Kizilsu Kirgiz Autonomous Prefecture was specific to plant species.These results provide a scientific basis for analyzing the mechanism of plant-microbe interactions and formulating sustainable management strategies for degraded grassland.