大黄鱼腺苷酸激酶基因ak1的克隆与表达分析OA

Adenylate kinase(AK)plays a crucial role in nucleotide metabolism and energy metabolism.To explore the molecular characteristics and tissue distribution of the ak1 gene in Larimichthys crocea,the complete coding sequence(CDS)of the ak1 gene was cloned from Larimichthys crocea in this study.Bioinformatics software was employed to analyze its sequence features and evolutionary characteristics,and quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the distribution of the ak1 gene in various tissues of Larimichthys crocea,such as muscle and heart.The results showed that the CDS of the ak1 gene in large yellow croaker was 582 bp in length,encoding 193 amino acids with a relative molecular weight of 21.24 kDa.It contained 1 O-glycosylation site and 15 phosphorylation sites.The AK1 protein had a typical ADK domain,existed as a monomer,and consisted of 9 α-helices and 5 β-sheets.Phylogenetic analysis revealed that the Ak1 of Larimichthys crocea had the closest genetic relationship with that of Dicentrarchus labrax.The expression level of the ak1 gene in muscle tissue was significantly higher than that in other tissues(P<0.05),followed by heart and skin tissues.This study provides a reference for elucidating the role of the ak1 gene in energy metabolism of Larimichthys crocea.