基于非靶向代谢组学的卷烟制品代谢物差异分析OA

To investigate the differences in metabolic components of cigarettes,type Ⅰcigarettes(100HHL96,100HTXC,100HTXX,100TY,100ZH)and type Ⅱ cigarettes(10DQM,10HJY,10XSD,35FRW,35HEH)were used as materials.Gas chromatography-mass spectrometry(GC-MS)and liquid chromatography-mass spectrometry(LC-MS)were employed to analyze the metabolic components of the two types of cigarette products,and metabolomic methods were used for heterogeneity analysis.The results showed that a total of 785 metabolic substances were identified across the 10 cigarette samples,with ketones(15%),esters and lipid molecules(10%),and terpenes(10%)ranking in the top three categories by structure,and ketones,alkaloids,and indoles ranking in the top three categories by content.In the comparison of two types of cigarette products,28 metabolites such as dimethyldodecylamine and L-pyroglutamic acid,and dihydrocelastryl diacetate showed significant differentiation.In type Ⅰ cigarettes,11 metabolites such as dimethyldodecylamine,L-pyroglutamic acid,and 5-hydroxymaltol were upregulated,while 17 metabolites such as dihydroquercetin diacetate,ethyl palmitate,and ethyl octadecanoate were downregulated.The main components affecting aroma,such as mesmin,methyl palmitate,and maltol,showed significant heterogeneity between the two types of cigarettes.This study provides a reference for improving cigarette processing technology and product quality.