朱砂根及其近缘种资源调查和品质评价OA

Objective To study the distribution and quality of the resources of A.crenata Sims and its closely re-lated species,and to lay the foundation for its sustainable development and utilisation.Methods This study was conducted after literature review and telephone consultation to determine the research locations and routes,using participant observation method,key person interviews and other methods to conduct the field research and collect samples.The agronomic trait indexes of the samples were determined with reference to the Chinese Pharmacopoe-ia,and the content of the samples of bergenin was determined by HPLC,and the principal component analysis method was used to screen the good germplasm resources.Results The research area covers 21 towns in 5 prefec-ture-level citis and 2 autonomous prefectures,with a predominantly mountainous terrain,all with shady slopes and low resource reserves.A total of 24 resource samples were collected(16 A.crenata Sims,5 A.crenata var.bicolor,and 3 A.crispa(Thunb.)A.DC.).For agronomic traits,only petiole length and abaxial leaf colour were significantly different(P＜0.05),while the rest of the traits were not significantly different.24 resource samples had low levels of bergenin,with only 43.75％of the roots,50％of the stems,and 31.25％of the leav-es of A.crenata Sims meeting the pharmacopoeia standards,while 100％of the roots,100％of the stems,and 20％of the leaves of A.crenata var.bicolor met the standards,and the percentage of A.crispa(Thunb.)A.DC.meeting the standards was zero.The top three composite rankings in the principal component analysis were A.crenata var.bicolor from Rongjiang County,Qiandongnan Prefecture,Guizhou Province,A.crenata Sims from Jinsha County,Bijie City,Guizhou Province,and A.Sims from Rongjiang County,Qiandongnan Prefecture,Guizhou Province.Conclusion The small amount of wild A.crenata Sims resources and its closely related spe-cies,and the low proportion of bergenin content that meets pharmacopoeia standards mean that a simultaneous in-crease in A.crenata Sims production and bergenin content is imminent,and the first place ranking of A.crenata var.bicolor in the principal component analysis provides strong evidence for its potential as a A.crenata Sims basalogen.