CTRP12调控平滑肌细胞表型转化在腹主动脉瘤中的功能研究OA

Objective This study aimed to investigate the role of C1q/TNF-related protein-12(CTRP12)in Ab-dominal Aortic Aneurysm(AAA)and its underlying molecular mechanisms,with a focus on its effects on inflam-matory factors,matrix metalloproteinases(MMPs),and VSMC phenotypic switching.Methods Serum samples were collected from AAA patients and healthy volunteers,and CTRP12 levels were measured by ELISA.An AAA model was established in ApoE-/-mice via Angiotensin Ⅱ(Ang Ⅱ)infusion using osmotic minipumps.Adeno-associated virus was administered via tail vein injection to modulate CTRP12 expression.Pathological changes in the abdominal aorta were assessed by hematoxylin-eosin(HE)and Masson staining.The expression levels of CTRP12,inflammatory factors(IL-6,IL-1β,TNF-α),MMPs(MMP2,MMP9),and VSMC pheno-typic markers in the abdominal aorta of AAA mice were analyzed using qPCR and Western blot.Results CTRP12 expression was significantly elevated in the serum of AAA patients.In ApoE-/-mice,CTRP12 expression was markedly increased in both serum and abdominal aortic tissues,accompanied by elevated concentrations of IL-6,IL-1β,and TNF-α,and upregulation of MMP2 and MMP9.Knockdown of CTRP12 expression led to reduced levels of these inflammatory factors,downregulated MMP2 and MMP9 expression,and inhibited VSMC phenotyp-ic switching.Conclusion CTRP12 may accelerate AAA progression by regulating inflammatory responses,MMPs expression,and VSMC phenotypic switching.