异氟烷对中央杏仁核CRH神经元的激活效应及其空间分布特征研究OA

Objective The aim of this study was to investigate the isoflurane-activated cells in the central amyg-dala(CeA)and their relationship with corticotropin-releasing hormone(CRH)neurons.Methods Eight healthy male CRH-tdTomato-Cre mice were randomly assigned to two groups:the isoflurane group(1.4％isoflurane an-esthesia for 2 hours,n=4)and the air control group(n=4).After the experiment,cardiac perfusion was per-formed to collect the brains,and frozen sections of the CeA region were prepared.Immunofluorescence staining was then carried out,and fluorescence microscopy was used for observation and image acquisition.The number of isoflurane-activated cells and the distribution and co-localization of CRH neurons in different layers of the CeA were statistically analyzed.Results Compared to the air control group,the isoflurane group showed a significant increase in neuronal activity in the CeA,with a notable increase in c-Fos-positive cells(P＜0.05).Some of the isoflurane-activated cells were CRH neurons,but the co-localization rate was low.The activated cells were pri-marily located in the posterior regions of the CeA,while CRH neurons were predominantly located in the anterior regions of the CeA.Conclusion Isoflurane significantly activates neurons in the CeA,but only a small proportion of these activated cells are CRH neurons,with most of the activated cells not being CRH neurons.