三七全株不同药用部位鲜品与干品蛋白质的特征研究OA

Objective:To explore the application value of proteins from different medicinal parts of Panax notoginseng for the identification of corresponding medicinal materials.Methods:The total crude protein was extracted from the fresh and dried samples of underground parts(main root,rootlets,and rhizomes)and aboveground parts(stems and leaves,flowers)and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE).The protein candidates for multidimensional identification were selected by mass spectrometry combined with liquid chromatography.Results:The extraction rate of total crude protein from fresh medicinal parts of P.notoginseng was higher than that from corresponding dried medicinal parts,with some non-significant differences.The fresh samples showed no significant differences among different producing areas,except the main root and the stems and leaves of P.notoginseng from Wenshan and Qujing(P<0.05).There were significant differences in the protein electrophoresis profiles of fresh samples between the underground and aboveground parts.The bands at 60 kDa and 25～35 kDa were specific to fresh and dried underground parts,while they were absent for fresh or dried aboveground parts.The specific protein bands of underground parts from P.notoginseng were similar to those of P.ginseng.The results of mass spectrometry combined with liquid chromatography showed that the two specific protein bands from P.notoginseng and P.ginseng were RNase-like storage proteins.Moreover,the amino acid sequence comparison of RNase-like storage proteins from the underground parts of P.notoginseng and P.ginseng showed 6 varied amino acid sites.Conclusion:The underground and aboveground parts of P.notoginseng can be identified by the protein profile,and the significant characteristic bands are mainly RNase-like proteins.