内蒙古阴山地区栽培小秦艽的品质评价OA

Objective:To evaluate the quality of wild and cultivated Gentiana dahurica from Yinshan area of Inner Mongolia.Methods:Morphological characterization and thin-layer chromatography were employed to identify G.dahurica.High performance liquid chromatography(HPLC)was employed to determine the content of loganic acid and gentiopicroside.Liquid chromatography-mass spectrometry was employed to characterize the main chemical components in G.dahurica,and principal component analysis and heatmap analysis were performed to analyze the obtained data.Results:During the growing process,the roots of G.dahurica gradually showed branches and darken skin,with ncreases in diameter and length.Thin-layer chromatography showed that the dark spots and color of cultivated and wild G.dahurica of 3 to 7 years old were consistent with the reference standard.The mass fractions of gentiopicroside and loganic acid in 14 batches of G.dahurica samples were 4.688 9%-8.531 2%and 0.233 2%-1.152 7%,respectively.The total mass fractions of gentiopicroside and loganic acid in these samples were 5.379 7%-9.043 8%.The content of the two compomemts in the samples from all the plots met the requirements of the Chinese Pharmacopoeia(2020 version).The chemical composition of G.dahurica from different growth years was analyzed,which revealed a total of 20 common components,predominantly iridoids.Furthermore,relative quantification was conducted for these components.With the extension of growing time,the accumulation of active components in G.dahurica increased year by year,while the increase slowed down after 5 years of cultivation.Different planting methods and planting densities had little effect on the quality of G.dahurica.Conclusion:Yinshan area of Inner Mongolia is suitable for the cultivation of G.dahurica.It is suggested that G.dahurica of 4-5 years should be harvested and processed in the producing area,and the obtained medicinal materials demonstrate high quality.