贝莱斯芽孢杆菌对芒果炭疽病的抑制作用及果实保鲜效果OA
The Inhibitory Efficacy of Bacillus velezensis Against Mango Anthracnose and Its Influence on Fruit Fresh Preservation
炭疽病是芒果(Mangifera indica)主要病害之一,由胶孢炭疽菌(Colletotrichum gloeosporioides)侵染引起.发病后影响果实品质,从而缩短果实货架期.该研究从生物防治角度出发,利用贝莱斯芽孢杆菌(Bacillus velezensis)和从芒果果实中分离出的炭疽菌进行实验,以贝莱斯芽孢杆菌菌悬液(CB)处理芒果果实,并利用平板对峙法观察CB对胶孢炭疽菌的抑制效果.用发酵液(CF)处理胶孢炭疽菌,结果显示发酵液能够有效抑制胶孢炭疽菌孢子萌发并缩短芽管长度,显著抑制菌丝生长,其中CF浓度为2%和4%时,对炭疽菌菌丝抑制率分别为75.18%和80.96%.果实活体接种实验表明,CB和CF能有效抑制果实病斑直径增大,抑制率分别为44.33%和65.00%;与对照组相比,处理组果实可溶性固形物含量显著降低,可滴定酸、果实总酚和类黄酮含量均高于对照,且发酵液处理未对果实品质产生负面影响.上述结果表明,贝莱斯芽孢杆菌菌株能够抑制芒果炭疽病的发生,在芒果保鲜方面有较大的应用潜力.
INTRODUCTION:Anthracnose,primarily caused by Colletotrichum gloeosporioides is a main disease affecting man-goes,leading to significant postharvest losses by deteriorating fruit quality and reducing shelf life. RATIONALE:Addressing postharvest anthracnose is a critical challenge in the mango industry.Biological control me-thods,such as utilizing antagonistic bacteria,offer sustainable alternatives to chemical treatments.This study investi-gates the efficacy of Bacillus velezensis in inhibiting C.gloeosporioides and its potential in preserving mango fruit quality. RESULTS:The application of B.velezensis culture filtrate(CF)effectively inhibited spore germination and mycelial growth of C.gloeosporioides.At CF concentrations of 2%and 4%,mycelial inhibition rates were 75.18%and 80.96%,respectively.In vivo experiments demonstrated that both bacterial suspension(CB)and CF treatments significantly re-duced lesion expansion on mangoes,with inhibition rates of 44.33%and 65.00%,respectively.Treated fruits exhibited a slower decrease in titratable acids and maintained higher levels of total phenols and flavonoids,indicating delayed ri-pening and extended shelf life. CONCLUSION:B.velezensis exhibits strong antagonistic activity against C.gloeosporioides,effectively controlling mango anthracnose and preserving fruit quality.Its application as a biocontrol agent holds promise for sustainable postharvest management in mango production.
曹慧;羊尾燕;纳琦婷;朱长松;孟兰环;宋海超;史学群
海南大学食品科学与工程学院,海口 570228海南大学生命健康学院,海口 570228海南大学食品科学与工程学院,海口 570228海南大学食品科学与工程学院,海口 570228海南大学食品科学与工程学院,海口 570228海南大学生命健康学院,海口 570228海南大学生命健康学院,海口 570228
贝莱斯芽孢杆菌生物防治胶孢炭疽菌芒果果实保鲜
Bacillus velezensisbiological controlColletotrichum gloeosporioidesmangofresh preservation
《植物学报》 2026 (1)
68-77,10
海南省重点研发项目(No.ZDYF2025XDNY092)
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