藜麦LOC110717159基因克隆及功能分析OA

INTRODUCTION:The glycoside hydrolase(GH)gene family plays a crucial role in regulating plant grain size. RATIONALE:To elucidate its biological role during grain filling,we cloned and functionally characterized LOC110717-159,a GH gene in quinoa,through bioinformatics analysis,spatiotemporal expression profiling,and heterologous ex-pression experiments in Arabidopsis. RESULTS:LOC110717159 has a 1 023 bp coding sequence(encoding 339 amino acids)in both germplasms,differing by a single nucleotide.Analysis of the～2 000 bp LOC110717159 promoter identified 119 cis-elements,including three hormone-responsive elements(MeJA,SA,and GA).Phylogenetically,it is closely related to Beta vulgaris,Spinacia oleracea and Amaranthus tricolor.qRT-PCR revealed high expression of LOC110717159 in large-grained quinoa at 21 days after anthesis(21 DAA).Arabidopsis overexpression lines showed significantly reduced 1 000 grain weight(P＜0.05)but no change in grain length and width.Haplotype analysis of 17 quinoa germplasms indicated that 62.5％of large germplasms(≥4.0 g/1 000 grains)carried the large-grained haplotype,while 77.8％of small germplasms(≤3.0 g)shared the small-grained haplotype. CONCLUSION:In conclusion,LOC110717159 likely functions as a negative regulator in quinoa grain filling.