岩白菜TCP基因家族的表达模式及代谢关联分析OA

INTRODUCTION:The TCP protein family is a plant-specific group of transcription factors known to regulate key biological processes,including growth and development,as well as stress responses.Despite their critical roles,the TCP gene family in Bergenia purpurascens remains uncharacterized.This study aims to systematically identify and analyze the BpTCP gene family in B.purpurascens using transcriptome-based bioinformatics approaches,providing new insights into their potential functions in cold adaptation and secondary metabolism. RATIONALE:B.purpurascens exhibits remarkable resilience to abiotic stresses,particularly cold,and contains abundant secondary metabolites.Given the documented roles of TCP genes in stress responses and metabolic regulation in other plants,we hypothesized that BpTCP genes may contribute to these traits.A comprehensive analysis of this gene family could reveal novel mechanisms underlying stress adaptation and metabolite synthesis,supporting future genetic im-provement and biotechnological applications. RESULTS:Through transcriptome-based bioinformatics analysis,we identified 16 BpTCP genes in B.purpurascens,which were phylogenetically classified into two major groups,with all members containing conserved TCP domains and closely related proteins sharing similar motif patterns.Tissue-specific expression profiling revealed distinct spatial ex-pression patterns across different tissues,suggesting functional diversification among family members.Notably,partial genes,including BpTCP10,BpTCP1 and BpTCP12,exhibited significant expression changes under cold stress,implying their potential cold-responsive roles.Furthermore,expression levels of specific BpTCP genes correlated significantly with accumulation of various secondary metabolites,particularly flavonoids and phenolics,suggesting their regulatory in-volvement in metabolic pathways. CONCLUSION:This study provides the first genome-wide characterization of the BpTCP gene family in B.purpurascens,demonstrating its potential roles in growth and development as well as cold stress response,and secondary metabolism.The differential expression of BpTCP genes under stress and their correlation with metabolite levels lay a foundation for future functional studies.