灰毡毛忍冬根腐病根际土壤理化性质及真菌群落特征OA

To investigate the effect of root-rot disease occurrence on physicochemical properties and fungal community structure diversity in rhizosphere soil of Lonicera macranthoides,high-throughput sequencing technology was employed to compare and analyze the changes on fungal community structure and diversity in the rhizosphere soil of root-rot diseased and non-diseased'Yulei No.1'plants.Soil physicochemical characteristics,including content of soil organic matter,available phosphorus,available potassium,total nitrogen,total phosphorus,total potassium,ammonium nitrogen,nitrate nitrogen and pH,were measured and analyzed to explore the correlation between soil physicochemical properties,fungal community structure,and the occurrence of root rot disease in L.macranthoides.The results showed that after infection with root rot disease,the pH value of the rhizosphere soil of L.macranthoides was significantly lower than that of the healthy group(P＜0.05),the available phosphorus and total phosphorus content were significantly higher than those of the healthy group,and the available potassium and total potassium content were lower than those of the healthy group.The fungal richness index in the rhizosphere soil of the diseased groups in the three planting bases was significantly lower than that of the healthy group(P＜0.05),the relative abundance of Ascomycota was lower than that of the healthy group,the relative abundance of Basidiomycota was higher than that of the healthy group,and the relative abundance of Fusarium was significantly higher than that of the healthy group(P＜0.05).Correlation analysis showed that total phosphorus had the most significant impact on the fungal community structure in rhizosphere soil,followed by nitrate nitrogen.Fusarium was significantly positively correlated with total phosphorus.This study indicates that the decrease in soil potassium content,pH,fungal community richness,and increase in phosphorus and nitrogen content,harmful fungi such as Fusarium species are important reasons for the occurrence of root rot disease in the diseased group of L.macranthoides in three planting bases.This study provides a scientific basis for the research on the occurrence mechanism and prevention and control techniques of root rot disease in L.macranthoides.