基于纤维素纳米晶与表面活性剂的Pickering乳液增强对柑橘叶片的亲和性OA

Cellulose nanocrystal(CNC)-based Pickering emulsions(PE)possess great potential for agricultural pest management owing to their high stability and biocompatibility.In this study,carvacrol(CAR)was selected as the antibacterial active ingredient to prepare a CNC/surfactant synergistically stabilized CAR-loaded Pickering emulsion(CAR@PE),and its biological activity against Xanthomonas citri subsp.citri(Xcc)was evaluated.CNC was characterized by transmission electron microscopy(TEM)and X-ray diffraction(XRD).The optimal formulation was screened out using the creaming index(CI),and the emulsion properties were examined in terms of environmental stability,rheological property,and leaf surface interaction.The results showed that the CNC used was nanoscale rod-like crystals belonging to cellulose type Ⅰ.The CAR@PE containing surfactant SK-551 and CNC exhibited a CI of 0,indicating excellent stability without phase separation.The emulsion maintained good stability under various temperature,pH and ionic strength conditions.CAR@PE behaved as a non-Newtonian fluid with shear-thinning characteristics and underwent phase transitions at high frequencies.Its surface tension was 28.70 mN/m,and the contact angle was 27.94°,demonstrating superior spreading performance and a deposition rate of 24.94％on citrus leaves.The EC50 value against Xcc was 24.28 mg/L.These findings broaden the application of Pickering emulsions in agricultural disease prevention and control,reveal the bioactivity of CAR against Xcc,and provide a novel strategy for controlling citrus canker disease.