通过斑马鱼模型探究多酚类物质抗辣木叶蛋白致敏作用OA

To explore the allergenic effects of Moringa oleifera leaf protein(MLP)and the anti-allergic effects of polyphenols such as lotus leaf polyphenol extract(LPE)and Polygonatum polyphenol extract(PPE),MLP was extracted to construct a zebrafish food allergy model.Zebrafish were simultaneously administered LPE and PPE.The allergenic effects of MLP and the anti-allergic effects of LPE and PPE were investigated by behavioral analysis,intestinal section staining,and real time fluorescent quantitative polymerase chain reaction(qRT-PCR).The results indicated that MLP reduced the locomotor activity of zebrafish,while LPE and PPE could restore this indicator,demonstrating a protective effect.MLP caused significant damage to the intestinal barrier,whereas LPE and PPE enhanced immune responses,promoted intestinal recovery and alleviated allergic symptoms.qRT-PCR result showed that LPE and PPE regulated the expression of inflammatory factors such as interleukin-6(IL-6),interleukin-1β(IL-1β),interleukin-4/13B(IL-4/13B),and interleukin-10(IL-10),thereby inhibiting the inflammatory response induced by MLP.This study confirms the allergenicity of MLP and the anti-allergic potential of LPE and PPE,providing a scientific basis for the prevention and treatment of food allergies.