芍药组(Sect.Paeonia)种间远缘杂交花粉萌发与花粉管生长观察OA

In this study,we carried out an interspecific distant hybridization experiment with Chinese Paeonia lactiflora as female parent and P.intermedia,P.obovata,P.veitchii and P.mairei as male parents,aiming to screen the best hybrid combinations.Pollen germination and pollen tube growth of the male parents of both close hybridization and distant hybridization were observed.The results are summarized as follows:1)three hybrid combinations with high seed-setting rates were identified,namely'Miss America'×P.veitchii,'Miss America'×P.obovata,and'Lian Tai'×P.obovata.Additionally,two hybrid combinations,'Miss America'×P.obovata and'Karl Rosenfield'×P.obovata,with a higher number of effective seeds and seedling emergence rates,were selected.2)The status of pollen tube growth within 1-48 h post-pollination was closely observed.It was confirmed that the primary cause of incompatibility in distant hybridization was the callose reaction in the stigma.This study provides a theoretical basis of cytology and germplasm materials for the cultivation of new ornamental peony varieties.