表里井穴刺血法对脂多糖致热家兔前列腺素E2水平的影响OA

Objective:To explore the antipyretic mechanism of bloodletting therapy at exterior-interior Jing acu-points.Methods:30 male rabbits were randomly divided into normal group,model group,Shaoshang group,Shang-yang group and Shaoshang-Shangyang group.An exogenous fever model was established by intravenous injection of lipopolysaccharide(LPS).After successful modeling,bloodletting therapy was performed at bilateral Shaoshang acupoint,bilateral Shangyang acupoint,and bilateral Shaoshang plus Shangyang acupoint respectively.The anti-pyretic mechanism was explored by analyzing the levels of prostaglandin E2(PGE2)and cyclooxygenase-2(COX-2)in serum and brain tissue of rabbits in each group after intervention.Results:Compared with the model group,the anal temperature of rabbits in the Shaoshang group,Shangyang group and Shaoshang-Shangyang group de-creased with statistical significance(P＜0.01).Compared with the normal group,there was no statistical difference in the anal temperature of rabbits in the Shaoshang group,Shangyang group and Shaoshang-Shangyang group(P＞0.05).Compared with the model group,the serum PGE2 level of rabbits in the Shaoshang group decreased with statistical significance(P＜0.05),and that in the Shaoshang-Shangyang group decreased with significant statistical significance(P＜0.01).The brain tissue PGE2 level of rabbits in the Shangyang group decreased with significant statistical significance(P＜0.01),and that in the Shaoshang-Shangyang group decreased with statistical significance(P＜0.05).The hypothalamic COX-2 level of rabbits in the Shaoshang group decreased with significant statistical significance(P＜0.01),and that in the Shangyang group and Shaoshang-Shangyang group decreased with statisti-cal significance(P＜0.05).Conclusion:Bloodletting therapy at Shaoshang and Shangyang acupoint may exert an antipyretic effect by inhibiting the COX-2/PGE2 signaling pathway and reducing the release of central and periph-eral pyrogenic mediators.