山东省和广东省部分地区不同动物源大肠杆菌耐药及毒力基因检测OA
Detection of Antibiotic Resistance and Virulence Genes of Different Animal-Derived Escherichia coli in Selected Regions in Shandong and Guangdong Provinces
为探究我国南方和北方区域不同动物源大肠杆菌分离株耐药及毒力基因分布情况,从山东省和广东省多家养殖场采集疑似感染大肠杆菌的不同动物样品201份,进行大肠杆菌分离鉴定,并对分离株进行抗生素敏感性试验以及耐药和毒力基因检测.结果显示:共分离鉴定出193株大肠杆菌,分离率为96.02％,其中苍蝇源菌株占35.23％,蛋鸡源占29.02％,鹅源占24.35％,兔源和羊源各占4.66％,肉鸡源占2.07％;89.64％的菌株呈现多重耐药特性,对四环素、萘啶酸、甲氧苄啶的耐药率均高于70.00％,对阿米卡星(46.11％)、氟苯尼考(47.15％)等呈现中度耐药;对替加环素敏感(耐药率为1.04％);从分离鉴定的大肠杆菌中共检出28种耐药基因,其中floR(44.56％)、tetA(19.69％)和aadA1(16.58％)携带率较高,但具有重要临床意义的mcr1耐药基因携带率仅为9.33％;共检出16种毒力基因,其中phoA(33.16％)和ropS(31.09％)检出率较高.耐药率与毒力基因检出率在不同动物间存在差异,肉鸡源与苍蝇源菌株的耐药率均达100％,毒力基因检出率分别为75.00％和44.11％,而鹅源、羊源菌株的耐药率相对较低(70.21％、66.67％),毒力基因检出率较高(89.36％、88.89％).结果表明,分离自我国北方山东与南方广东两地的不同动物源大肠杆菌普遍呈现较强的耐药性,耐药基因和毒力基因多样且检出率较高.本研究揭示了多种动物源大肠杆菌的耐药及毒力基因分布情况,为当地抗菌药物使用方案制定和耐药性防控提供了数据支撑.
In order to investigate the distribution of antibiotic resistance and virulence genes of different animal-derived Escherichia coli(E.coli)in southern and northern China,a total of 201 samples were collected from different animals suspected of E.coli infection on several farms in Shandong Province and Guangdong Province,for isolation and identification of the strain,followed by antibiotic susceptibility testing and detection of antibiotic resistance and virulence genes.The results showed that a total of 193 strains of E.coli were isolated and identified,with an isolation rate of 96.02％,specifically,35.23％for fly-derived strains,29.02％for layer hen-derived strains,24.35％for goose-derived strains,4.66％for each rabbit-and sheep-derived strains,and 2.07％for broiler chicken-derived strains.89.64％of strains were resistant to multiple drugs,especially to tetracycline,nalidixic acid and trimethoprim,with the resistance rate above 70.00％,moderately to amikacin(46.11％)and flumequine(47.15％),but sensitive to tigecycline,with a resistance rate of 1.04％.28 antibiotic resistance genes were detected in the identified E.coli strains,the carriage rate was high in floR(44.56％),tetA(19.69％)and aadA1(16.58％),but 9.33％in mcr1 that was clinically significant;16 virulence genes were detected,with a high detection rate in phoA(33.16％)and ropS(31.09％).The resistance rate and the detection rate of virulence genes were different among different animals,the former was 100％,and the latter was 75.00％and 44.11％for broiler chicken-and fly-derived strains,respectively,while the former was lower(70.21％and 66.67％),and the latter was higher(89.36％and 88.89％)for geese-and sheep-derived strains.In conclusion,different animal-derived E.coli isolated from the two provinces generally presented strong antibiotic resistance,with diverse resistance and virulence genes and high detection rate.The distribution of antibiotic resistance and virulence genes in multiple animal-derived E.coli was revealed in the study,and served as a data support for future development of local antimicrobial stewardship programs and resistance control strategies.
柳妍玲;赵永达;段笑笑;原培勋;郭莉莉;付来宇;王群义;张一驰;李彦;王欢;宋亮;徐靖雯;陈佳文
青岛农业大学动物医学院,山东青岛 266109青岛农业大学动物医学院,山东青岛 266109||青岛博霖生物科技有限公司,山东青岛 266114青岛市动物疫病预防控制中心,山东青岛 266033山东省畜产品质量安全中心,山东济南 250102青岛农业大学动物医学院,山东青岛 266109||青岛博霖生物科技有限公司,山东青岛 266114青岛农业大学动物医学院,山东青岛 266109青岛易邦生物工程有限公司,山东青岛 266032青岛农业大学动物医学院,山东青岛 266109青岛市动物疫病预防控制中心,山东青岛 266033青岛博霖生物科技有限公司,山东青岛 266114青岛博霖生物科技有限公司,山东青岛 266114青岛农业大学动物医学院,山东青岛 266109青岛农业大学动物医学院,山东青岛 266109
农业科技
大肠杆菌不同动物源耐药基因毒力基因
E.colidifferent animal-deriveddrug resistance genevirulence gene
《中国动物检疫》 2026 (1)
24-33,10
山东省重点研发计划项目(2024CXGC010910)山东省泰山产业领军人才项目(TS20220701)中央引导地方资金项目(YDZX2023123)青岛市产业培育计划科技惠民专项(24-1-8-xdny-4-nsh,25-1-5-xdny-30-nsh)
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