2022-2023年豫东地区病死鸡群主要病毒核酸检测OA

In order to investigate the prevalence of major viral diseases in chickens in Eastern Henan,a total of 225 samples were collected from 61 farms/households from 2022 to 2023,and tested for nucleic acids of avian infectious anemia virus(CIAV),infectious bronchitis virus(IBV),H9N2 subtype avian influenza virus(H9N2AIV),fowl adenovirus(FAdV),infectious bursal disease virus(IBDV),Newcastle disease virus(NDV),and infectious laryngotracheitis virus(ILTV),based on which,statistical analysis was conducted.The results showed that the overall detection rates for CIAV,IBV,H9N2 AIV,IBDV,FAdV,NDV and ILTV were 48.44％,24.00％,8.00％,7.11％,7.11％,0.89％,and 0.89％,respectively,that for CIAV was increasing and reached 53.10％in 2023,obviously higher than that in 2022(P＜0.05),that for FAdV was decreasing and greatly lower in 2023 compared to that in 2022(P＜0.05).Co-infections were observed,with a co-detection rate of 4.44％for H9N2 AIV and IBV,and 6.22％for CIAV and FAdV.All detected H9N2 AIV strains belonged to h9.4.2.5 clade,with 88.89％(16/18)classified into h9.4.2.5c subclade.5 serotypes within four genogroups of FAdV were detected,in particular,FAdV-4 being the most prevalent at 37.50％(6/16).For IBV,QX-like strain(GI-19 lineage)was dominant,accounting for 64.81％(35/54).In conclusion,multiple viral strains were widely prevalent in chickens in the region,posing high risk of co-infection,in particular,CIAV and IBV with severe epidemiological status,and H9N2 AIV and IBV with clear dominant strains,which should be specifically monitored,prevented and controlled.Farms/households were recommended to control any introduction of chickens and to implement biosecurity measures,to conduct regular disease testing and to evaluate the efficacy of vaccination,and thereby to effectively control any spread or prevalence of the above pathogens.