牦牛PSTPIP2和SLC6A18基因多态性与免疫性状的关联分析OA

PSTPIP2 and SLC6A18 genes play an important role in innate autoimmunity.In order to investigate the asso-ciation between the polymorphisms at the g.43165308C＞T locus of PSTPIP2 gene and the g.8294767T＞C locus of SLC6A18 gene with immune traits in yaks,in this study,the PSTPIP2 gene g.43165308C＞T locus and the SLC6A18 g.8294767 T＞C locus were amplified by PCR in 189 Niangya yaks,and using the PARMS SNP genoty-ping.The correlation between different gene loci and immune traits such as immunoglobulin,inflammatory mar-kers,and cytokines in yaks was analyzed using IBM SPSS Statistics 26.0 software.The results showed that there were CC,CT,and TT genotypes at the g.43165308C＞T locus of PSTPIP2 gene,and the dominant allele and genotype were C and CC,respectively,with frequencies of 0.720 and 0.534,respectively.The PSTPIP2 gene g.43165308C＞T locus was moderately polymorphic and in Hardy-Weinberg equilibrium(P＞0.05).There were three genotypes of CC,TC and TT at the g.8294767T＞C locus of SLC6A18 gene,and the dominant allele and genotype were T and TT,respectively,with frequencies of 0.796 and 0.660,respectively.The g.8294767T＞C locus of SLC6A18 gene was a moderate polymorphism and was in a Hardy-Weinberg imbalance state(P＜0.05).The results of association analysis showed that the g.43165308C＞T locus of PSTPIP2 gene was significantly correlated with IgA,IgM,CRP,IL2,IL4,IL6 and TNF-α in yak(P＜0.05).The g.8294767T＞C locus of SLC6A18 gene was significantly cor-related with CRP,IL4 and IFN-γ(P＜0.05).In summary,the polymorphisms of PSTPIP2 and SLC6A18 genes were significantly related to yak immune traits,and the two genes may be potential candidate genes affecting yak immune traits.