基于同期发情处理岷县黑裘皮羊血液生殖激素的变化分析OA

This study aimed to investigate the changes in peripheral blood reproductive hormone concentrations before and after synchronized estrus treatment in primiparous and multiparous Minxian Black fur sheep during the non-breeding season.In this study,progesterone vaginal suppositories(CIDR)+pregnant mare serum gonadotropin(PMSG)were used to treat 30 primiparous(group A)and 30 multiparous(group B)Minxian Black fur sheep with estrus at the same time,and 10 experimental ewes were randomly selected in each group to collect peripheral blood during the trial,and the concentrations of gonadotropin-releasing hormone(GnRH),follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2)and progesterone(P4)in peripheral blood were measured by ELISA.The results showed as follows:①The estrus rates of ewes in groups A and B were 60.00％and 83.33％respectively,group B significantly higher than group A(P＜0.05).The concentration patterns of E2 and P4 in the peripheral blood of groups A and B were consistent,while the changes in GnRH,LH,and FSH showed significant differences.Group B had higher concentra-tions of GnRH,E2,and P4 than group A,and lower FSH concentration than group A,but all differences were not signifi-cant(P＞0.05).And LH concentrations no significant difference between the two groups(P＞0.05).②The LH concentra-tion in the blood of both groups of experimental sheep reached its maximum value at 48 h after CIDR removal,signifi-cantly higher than the concentrations at the time of CIDR insertion and removal(P＜0.05).The E2 concentration reached its maximum value at 42 h after CIDR removal,significantly higher than the concentration at the time of CIDR inser-tion(P＜0.05).The P4 concentration significantly decreased at 42 h after CIDR removal(P＜0.05).Although FSH con-centration increased at 48 h after CIDR removal,the difference was not significant(P＞0.05).In conclusion,this study found that the success rate of estrus in multiparous sheep was significantly higher than that of primiparous sheep under the same conditions,and there was a significant difference in reproductive hormone secretion between primiparous sheep and multiparous sheep during estrus during the same period,and according to hormonal changes,it was found that 24-48 h after sponge removal was the concentrated estrus and ovulation time of Minxian Black fur sheep.