卵泡液HCY水平对PCOS患者颗粒细胞氧化应激和凋亡的影响OA

Objective:Discuss the impact of follicular fluid HCY on oxidative stress,apoptosis of granulosa cells,and clinical treatment outcomes in patients with PCOS.Methods:This study collected granulosa cells and follicular fluid from 160 patients who underwent IVF/ICSI-ET at our center from September 2023 to September 2024.The level of HCY in follicular fluid was measured by ELISA.According to whether the patients had PCOS and the level of HCY in follicular fluid,they were divided into four groups:PCOS+HCY>10μmol/L(Group A),PCOS+HCY≤10μmol/L(Group B),non-PCOS patients+HCY>10μmol/L(Group C),and non-PCOS patients+HCY≤10μmol/L(Group D).Flow cytometry was used to detect ROS levels in granulosa cells.Real-time PCR was performed to measure the expressions of SOD1,SOD2,CAT,and GPX4 in granulosa cells.Results:The ROS level showed a decreasing trend across the four groups,with Group A being higher,but there were no statistically significant differences among the groups(P>0.05).The expression level of SOD2 in Group A was signifi-cantly higher than that in the other three groups(P<0.05).The expression levels of CAT and GPX4 were in the order of Group A>Group B>Group C>Group D(P<0.05).Group C had the lowest expression levels of SOD1 and SOD2(P<0.05).The expression level of SOD1 in Group D was significantly higher than that in the other three groups(P<0.05).There were no inter-group differences in the rates of high-quality embryos,embryo implantation,clinical pregnancy,live birth,gestational hypertension,premature birth,and newborn weight(P>0.05).Conclu-sion:Elevated levels of HCY in follicular fluid can lead to compensatory disturbances in the an-tioxidant system of granulosa cells,but the long-term effects on IVF/ICSI-ET treatment remain to be demonstrated.