卷曲乳杆菌及徳氏乳杆菌上清液对阴道加德纳菌及大肠埃希菌的作用OA

Objective:To investigate the inhibitory effects of Lactobacillus crispatus(L.crispatus)supernatant,Lactobacillus delbrueckii(L.delbrueckii)supernatant,and their mixture on the bacterial counts and biofilms of Gardnerella Vaginalis(G.vaginalis)and Escherichia Co-li(E.coli).Methods:Five strains of L.crispatus isolated from vaginal secretions and L.del-brueckii isolated from Live Lactobacillus Capsule for Vaginal Use were selected.Biofilm forming capacity was determined using the Crystal Violet(CV)method.The supernatants from the cul-tures of L.crispatus,L.delbrueckii,and their mixture were prepared and co-cultured with G.vag-inalis and E.coli.The effects of these Lactobacillus on the bacterial counts,biofilm forming ca-pacity,and biofilm thickness of G.vaginalis and E.coli were measured using OD values,CV method,and Live/Dead staining followed by confocal laser scanning microscopy.Results:All five strains of L.crispatus and L.delbrueckii exhibited the strongest biofilm forming ability after 48 hours(P<0.05).L.crispatus had no significant inhibitory effect on the growth of G.vaginalis but strongly inhibited its biofilm formation(P<0.01).It did not inhibit the growth of E.coli but promoted the formation of its biofilm.L.delbrueckii inhibited the growth of G.vaginalis and E.coli(P<0.01),with a stronger inhibitory effect on E.coli than L.crispatus(P<0.05).It al-so inhibited the biofilm formation of G.vaginalis(P<0.05)but promoted the biofilm formation of E.coli.There is a synergistic effect between L.crispatus and L.delbrueckii,which enhances the growth and biofilm formation of E.coli.Conclusion:L.crispatus has a strong inhibitory effect on biofilm formation by G.vaginalis but no significant inhibitory effect on E.coli.L.delbrueckii can inhibit the growth and biofilm formation of G.vaginalis.There is a synergistic effect between L.crispatus and L.delbrueckii.