外源SiNPs提升涝害胁迫下生姜生长及耐涝性OA

[Objective]Waterlogging is a critical environmental factor limiting crop yield and quality.Since silica nanoparticles(SiNPs)can improve plant tolerance to waterlogging stress,exploring the effects of exogenous SiNPs on the waterlogging re-sistance of ginger provides both practical and theoretical support for addressing waterlogging issues in ginger cultivation.[Meth-ods]In this study,potted ginger seedlings from Shandong were used as experimental materials,and a total of 4 treatments were set up:normal water management(CK),foliar spraying of 200 mg/L SiNPs solution under normal water management(SiNP200),waterlogging(WL),and foliar spraying of 200 mg/L SiNPs solution prior to waterlogging(SiNP200+WL).The study investigated the effects of SiNPs on root configuration,leaf morphology,photosynthesis,water status,relative membrane permeability,reactive oxygen species(ROS)and osmotic adjustment substance in ginger under waterlogging stress.[Results]The results showed that waterlogging stress inhibited the root growth of ginger.Compared with CK,the total root length and root surface area in the WL treatment significantly decreased by 65.03%and 64.03%respectively.At the same time,the photosynthetic pigment content and net photosynthetic rate significantly decreased by 72.85%,and leaf relative water content dropped by 16.40%,leading to leaf yellowing and curling.In addition,waterlogging increased ROS levels in leaves,weakening cell membrane integrity.However,the SiNP200 treatment improved root growth under waterlogging.Compared to WL,total root length increased by 62.92%.SiNP200 mitigated the decline in photosynthetic pigments.Chlorophyll a and b contents increased by 44.44%and 40.00%,respectively,and the net photosynthetic rate increased by 108.63%.This en-hanced light energy utilization and promoted photosynthesis,reducing leaf yellowing.Additionally,SiNP200 improved leaf wa-ter status and increased leaf osmotic potential to maintain osmotic balance.It also reduced the accumulation of superoxide anion(O2-)and hydrogen peroxide(H2O2)in ginger leaves,further reducing oxidative damage and maintaining membrane integrity.[Conclusion]In conclusion,foliar application of SiNPs can improve root growth,maintain stable plant water content,enhance photosynthesis,and reduce oxidative damage in ginger under waterlogging stress,thereby improving overall waterlogging toler-ance.