和元畅络生血方对肾性贫血大鼠的治疗作用及其机制OA
Therapeutic effect and mechanism of Heyuan Changluo Shengxue Formula on renal anemia in rats
目的:探讨和元畅络生血方对腺嘌呤所致慢性肾衰竭(CRF)肾性贫血大鼠的治疗作用及其机制.方法:将33只SD大鼠按随机数字表法分为空白对照组(9只)和腺嘌呤造模组(24只),其中腺嘌呤造模组以腺嘌呤(200 mg/kg)灌胃造模4周诱导肾性贫血大鼠模型,造模成功后采用随机数字表法将其分为模型组、西药组和中药组,每组6只.西药组大鼠给予重组人促红细胞生成素180 U/(kg·周);中药组大鼠在西药组治疗基础上加用和元畅络生血方3.0 g/(kg·d)药液灌胃,其余组大鼠予以同等量生理盐水.所有大鼠均给药8周.给药结束后检测4组大鼠红细胞(RBC)、血红蛋白(Hb)、红细胞比容(HCT)、血清尿素氮(BUN)、肌酐(SCr)及促红细胞生成素(EPO)浓度,苏木精-伊红(HE)染色镜下观察肾脏病理改变.结果:HE染色结果显示,中药组和西药组大鼠肾脏病理损伤较模型组明显改善,且中药组恢复效果优于西药组.与空白对照组比较,模型组、西药组、中药组大鼠RBC、Hb、HCT及血清EPO水平均明显降低,血清BUN、SCr水平均明显升高,差异均有统计学意义(P<0.05).与模型组比较,西药组与中药组大鼠血清BUN、SCr水平较模型组明显降低,RBC、Hb、HCT及血清EPO水平明显升高,差异均有统计学意义(P<0.05).与西药组比较,中药组大鼠血清BUN、SCr水平明显降低,RBC、Hb、HCT及血EPO水平明显升高,差异均有统计学意义(P<0.05).结论:和元畅络生血方可通过保护肾功能,提高血清EPO浓度,从而改善CRF大鼠肾性贫血状态.
Objective:To explore the therapeutic effect and mechanism of Heyuan Changluo Shengxue Formula on re-nal anemia in rats with adenine-induced chronic renal failure(CRF).Methods:Thirty-three SD rats were randomly di-vided into a blank control group(9 rats)and an adenine modeling group(24 rats)according to the random number table method.The rats in the adenine modeling group were gavaged with adenine(200 mg/kg)for 4 weeks to induce a rat model of renal anemia.After successful modeling,the rats were randomly divided into a model group,a western medicine group and a traditional Chinese medicine(TCM)group,with 6 rats in each group.Rats in the western medicine group received recombi-nant human erythropoietin(rHuEPO)at 180 U/(kg·week);rats in the TCM group received Heyuan Changluo Shengxue Formula(3.0 g/(kg·d))by gavage in addition to the western medicine treatment;rats in the remaining groups received an equal volume of normal saline.All rats were treated for 8 weeks.After treatment,red blood cell(RBC)count,hemoglobin(Hb)level,hematocrit(HCT),serum urea nitrogen(BUN),creatinine(SCr)and erythropoietin(EPO)concentrations were measured in the four groups.Renal pathological changes were observed under hematoxylin-eosin(HE)staining.Results:HE staining results showed that renal pathological damage in the TCM and western medicine groups was signifi-cantly improved compared with the model group,and the recovery effect in the TCM group was superior to that in the west-ern medicine group.Compared with the blank control group,RBC,Hb,HCT and serum EPO levels in the model,western medicine and TCM groups were significantly decreased,while serum BUN and SCr levels were significantly increased.The differences were statistically significant(P<0.05).Compared with the model group,serum BUN and SCr levels in the west-ern medicine and TCM groups were significantly decreased,while RBC,Hb,HCT and serum EPO levels were significantly increased.The differences were statistically significant(P<0.05).Compared with the western medicine group,serum BUN and SCr levels in the TCM group were significantly decreased,while RBC,Hb,HCT and serum EPO levels were signifi-cantly increased.The differences were statistically significant(P<0.05).Conclusion:Heyuan Changluo Shengxue Formula can improve renal anemia in CRF rats by protecting renal function and increasing serum EPO concentration.
王琳琳;高继宁;李康康;王涛;王浩洁;朱玲萍
山西中医药大学第三临床学院,山西 晋中 030619山西省中西医结合医院肾病科,山西 太原 030013山西省中西医结合医院肾病科,山西 太原 030013山西中医药大学第三临床学院,山西 晋中 030619山西中医药大学第三临床学院,山西 晋中 030619山西省中西医结合医院肾病科,山西 太原 030013
医药卫生
慢性肾衰竭肾性贫血和元畅络生血方
CRFrenal anemiaHeyuan Changluo Shengxue Formula
《山西中医药大学学报》 2026 (1)
23-27,50,6
山西省中医药管理局科研课题项目(2024ZYY2A012)
评论