斑茅割手密复合体杂交后代抗旱生理特性及抗旱种质筛选OA

The study aimed to investigate the physiological responses of intergeneric hybrid Erianthus arundinaceus×Saccharum spontaneum L offspring at the seedling stage under drought stress,to identify novel sugarcane germplasm resources with better drought stress tolerance.The results would provide a theoretical basis for breeding and utilizing drought-tolerant new sugarcane varieties.Thirteen hybrid progenies and the sugarcane variety ROC22 were used as the experimental materials in a barrel experiment.The contents of malondialdehyde(MDA),soluble sugars(SS),soluble protein(SP),proline(PRO),and the activities of peroxidase(POD)and superoxide dismutase(SOD),were measured in sugarcane seedling leaves under mild and severe drought stress,and control(normal watering).A comprehensive evaluation of drought tolerance in thirteen progenies and ROC22 was conducted based on drought resistance coefficients,membership function values,and the comprehensive drought tolerance evaluation index(D value),to screen and identify drought-tolerant sugarcane germplasm resources.Under different drought treatments,the content of SS,SP,PRO,MDA,and the activities of SOD and POD were elevated in the seedling leaves of hybrid progenies.Notably,under severe drought stress,the physiological indicators of AS08-2-28 were significantly higher than those of ROC22.Based on the D value,seven progenies exhibiting greater drought tolerance than ROC22 at the seedling stage were identified,ranked as follows:AS08-2-28>AS11-1-101>AS11-4-44>AS11-75-1>AS12-A6-21>AS12-A6-5>AS12-176-5.This study evalu-ated the drought tolerance of the hybrid progenies at the seedling stage and successfully screened seven sugarcane germplasm resources with strong drought resistance.The findings would provide a foundational basis for the future mining of drought-related genes and offer scientific guidance and experimental support for the effective utilization of intergeneric hybrid complex offspring in breeding new sugarcane varieties with enhanced drought tolerance.