五指毛桃中补骨脂素的提取研究OA

The experiment aimed to investigate the differences in psoralen content among different parts of Ficus hirta,as well as the effect of microbial treatment on its psoralen content.The study first examined the method for determining psoralen content in Ficus hirta.Subsequently,composite bacterial cultures,including Bacillus licheniformis and lactic acid bacteria,were used to ferment powdered root,stem,and leaf samples of Ficus hirta.High-performance liquid chromatography(HPLC)was employed to detect the psoralen content in different treated parts of Ficus hirta.The results revealed variations in psoralen content across different plant parts,with the content decreasing in the order of roots,leaves,and stems.Following microbial fermentation,there was a trend toward increased psoralen content.Specifically,the psoralen levels in the roots,stems,and leaves of the Bacillus licheniformis composite treatment group(Group A)were higher than those in the control group.Similarly,the psoralen content in the roots and leaves of the lactic acid bacteria composite treatment group(Group B)was also elevated compared to the control.The study indicates that both Bacillus licheniformis and lactic acid bacteria composite agents can promote the release of psoralen from Ficus hirta,with the Bacillus licheniformis composite showing a more pronounced effect than the lactic acid bacteria composite.