紫花苜蓿对盐碱地土壤理化性质和生物多样性的影响OA

In this study,16S rRNA high-throughput sequencing technology and the dry funnel separation method for soil animals were used to systematically analyze the effects of alfalfa(Medicago sativa L.)cultivation on the soil envi-ronment,animal communities,and microbial communities in saline-alkali soils.The results showed that compared with saline-alkali soils without alfalfa cultivation,the ac-tivities of soil dehydrogenase(DHA),sucrase(SC),ure-ase(UE),catalase(CAT),polyphenol oxidase(PPO),and invertase(AI),as well as the contents of soil total ni-trogen,available nitrogen,available phosphorus,and available potassium in saline-alkali soils with alfalfa cultivation were significantly increased(P＜0.05),while the soil pH value was significantly decreased(P＜0.05).These changes in the soil environment directly drove the reconstruction of microbial communities.Specifically,compared with saline-alkali soils without alfalfa cultivation,the relative abundances of bacteria such as Flavisolibacter,Lysobacter,Steroidobacter,Blastoca-tellaceae,and RB41,as well as fungi such as Curvularia,Laetisaria,Phaeomycocentrospora,Naganishia,and Knufia in saline-alkali soils with alfalfa cultivation were significantly increased(P＜0.05).In contrast,the relative abundances of bacteria such as Nitriliruptoraceae,Afifella,Tepidamorphus,Balneolaceae,and Nitrosococcus,as well as fungi such as So-diomyces,Deconica,Candida,Robillarda,and Sordaria were significantly decreased(P＜0.05).There was a significant correlation between the soil microbial communities and the soil animal communities.The relative abundances of animals be-longing to Opiliones and Microcoryphia were significantly negatively correlated with the relative abundances of bacteria such as Nitriliruptoraceae,Afifella,Tepidamorphus,Balneolaceae,and Nitrosococcus(P＜0.05),and significantly positively correlated with the relative abundance of Steroidobacter(P＜0.05).Additionally,the relative abundances of Opiliones and Microcoryphia animals were significantly negatively correlated with the relative abundances of fungi such as Sodiomyces,De-conica,Candida,and Robillarda(P＜0.05).These results indicate that the soil animal communities are involved in the regulation of the soil microbial communities,and the interaction between soil animals and microorganisms further affects en-vironmental factors.This ternary interaction network of environmental factors-microorganisms-animals collectively constitutes the ecological basis for the improvement of saline-alkali soils by alfalfa.This study provides a theoretical basis for the bio-logical improvement of saline-alkali soils.