江苏葫芦科作物上西瓜银斑驳病毒的侵染与分子鉴定OA

The watermelon silver mottle virus(WSMoV)is an important member of the Orthotospovirus genus.It is transmitted by thrips and can infect many crops including those in the families Cucurbitaceae and Solanaceae.To clarify the infection status,molecular characteristics and transmission sources of WSMoV on vegetable crops in Jiangsu province,218 samples of cucurbit crops suspected to be infected with WSMoV were collected from the main vegetable producing areas dur-ing 2023-2024.The pathogen was identified by RT-PCR,cloning and sequencing.The identified WSMoV sequences were compared with the amino acid sequences and base sequences of WSMoV registered in the National Center for Biotechnology Information database of the United States for similarity analysis and phylogenetic tree construction,and the possible trans-mission sources of WSMoV in Jiangsu were analyzed.The results showed that WSMoV infection was detected in 10 cucum-ber samples from Jiangning District of Nanjing,Haimen District of Nantong and Jiawang District of Xuzhou,with a virus carrying rate of 4.59%.The N genes of WSMoV iso-lates in the three regions of Jiangsu exhibited considerable divergence,and the similarity was only 91.91%.Among them,the sequences of Xuzhou Jiawang isolate(PV177960)and Nantong Haimen isolate(PV177961)were consistent,and the similarity with isolate WSMoV-SJZ(PQ073372)was higher,and they were clustered into the same branch in the phylogenetic tree.The Nanjing Jiangning iso-late(PV177962)was highly similar to the isolate THBC(PQ310671)and the isolate HNTG(OQ715365),and clustered into the same branch in the phylogenetic tree,indicating that the WSMoV infecting Jiangsu cucumbers may come from dif-ferent regions.This study provides the first report of WSMoV occurrence in vegetable crops in Jiangsu province,which is of great significance for the spread,prevalence and control strategy of the virus.