急性缺血性卒中患者血栓中巨噬细胞数量与中性粒细胞胞外陷阱网含量相关OA

Objective To explore the correlation between the number of macrophages and neutrophil extra cellular traps(NETs)in thrombi of patients with acute ischemic stroke(AIS),and to compare the NET-degrading and clearing abilities of macrophages in different polarization states.Methods Immunohistochemical staining was per-formed to detect CD16+macrophages,CD163+macrophages and citrullinated histone 3(CitH3)in AIS thrombi.Thrombi were co-cultured with macrophages,and the CitH3 concentration in the supernatant was measured to eval-uate the NET-degrading and clearing capacities of different macrophage subtypes.Results All 30 thrombi showed positive expression of CD16 macrophages,CD163 macrophages and CitH3.The numbers of both CD16+and CD163+macrophages were positively correlated with the percentage of CitH3-positive area(CD16:r=0.538,P＜0.05;CD163:r=0.641,P＜0.001).After co-culture,the CitH3 concentration was significantly lower in both the M1-and M2-macrophage groups than in the blank control group(M1:t=-3.981,P＜0.001;M2:t=-2.057,P＜0.05).The concentration of CitH3 was lower in the M1 group than in the M2 group(t=-2.125,P＜0.05).Conclusions Both M1 and M2 macrophage infiltration are correlated with NET content in AIS thrombi,and their abilities to clear and degrade NETs are different.