青石止痒软膏减轻咪喹莫特诱导的银屑病小鼠皮肤损伤OA

Objective To investigate the therapeutic effect and underlying mechanism of Qingshi anti-itch ointment(QAO)on imiquimod-induced psoriatic skin lesions in mice.Methods Mice were randomly divided into five groups:the control,model,low-dose QAO,high-dose QAO,and positive drug groups.Skin lesions were evaluated using the PASI scoring system,and histopathological changes were observed via HE staining.The protein levels of p65,IκBα,NLRP3,ASC,and caspase-1 in skin tissue were analyzed by Western blot.RT-qPCR was used to measure the mRNA expression of p65 and IκBα.Serum levels of IL-1β,IL-18,TNF-α,and IFN-γ were quantified by ELISA.Results Compared with the control group,the model group exhibited significant erythema,scaling,and skin thickening,accompanied by increased PASI scores,elevated pro-inflammatory cytokines(IL-1β/IL-18),upregulated NLRP3/ASC/caspase-1 proteins,and activated NF-κB signaling(increased p65 and decreased IκBα).Treatment with QAO and positive drug effectively reduced PASI scores,improved skin symptoms,sup-pressed NLRP3 inflammasome activation,and restored the NF-κB/IκBα balance(all P＜0.05).Conclusions QAO alleviates psoriatic skin lesions in mice by inhibiting the NF-κB/NLRP3 signaling axis,thereby reducing pro-inflammatory cytokine production and inflammatory responses.