基于"气血辨证"理论探讨白花蛇舌草对肝硬化大鼠的作用及对NF-κB表达纤维化的影响OA
An Investigation into the Effects of Hedyotis diffusa on Rats with Liver Cirrhosis Based on the Theory of Qi and Blood Differentiation and Its Influence on NF-κB Expression and Fibrosis
目的:基于"气血辨证"理论,探究白花蛇舌草对肝硬化大鼠肝脏组织纤维化及 NF-κB表达的影响.方法:将SD大鼠随机分为对照组、模型组、阳性药物组、白花蛇舌草低、高剂量组,每组 7只.腹腔注射四氯化碳构建肝硬化大鼠模型.HE染色检测肝脏组织的病理学改变,ELISA实验检测血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、IL-6、TNF-α 水平,Mas-son染色检测肝脏组织的纤维化程度,RT-qPCR 检测肝脏组织的 α-SMA、Col1a1、TGF-β1 mRNA 的表达,免疫组织化学染色实验检测肝脏组织中p65 NF-κB蛋白的表达与定位.结果:相对于对照组,模型组大鼠的肝脏组织呈现明显的肝硬化病理学改变,血清中 ALT、AST、TBIL、IL-6、TNF-α 升高(P<0.05),肝脏组织胶原纤维大量增生,肝脏组织中α-SMA、Col1a1、TGF-β1、p65 NF-κB 升高(P<0.05);相对于模型组,阳性药物组、白花蛇舌草低、高剂量组大鼠肝脏组织的病理学损伤改善,血清中 ALT、AST、TBIL、IL-6、TNF-α降低(P<0.05),肝脏组织胶原纤维增生减少,肝脏组织中α-SMA、Col1a1、TGF-β1、p65 NF-κB降低(P<0.05).结论:白花蛇舌草能够通过抑制炎症来减轻肝硬化大鼠肝脏组织的纤维化,其机制可能与抑制NF-κB信号活化有关.
Objective:To investigate the effects of Hedyotis diffusa on hepatic fibrosis and NF-κB ex-pression in rats with liver cirrhosis based on the theory of Qi and Blood Differentiation.Methods:The SD rats were randomly divided into a control group,a model group,a positive drug Yishanfu group,and Hedyotis dif-fusa low-and high-dose groups,with 7 rats in each group.Intraperitoneal injection of carbon tetrachloride was conducted to construct a rat model of liver cirrhosis.HE staining was used to detect pathological changes in liver tissue.ELISA assay was used to detect the levels of alanine aminotransferase(ALT),aspartate amin-otransferase(AST),total bilirubin(TBIL),IL-6,and TNF-α in serum.Masson staining was used to detect the degree of fibrosis in liver tissue.RT-qPCR was used to detect the mRNA expressions of α-SMA,Col1a1,and TGF-β1 in liver tissues.An immunohistochemical staining assay was used to detect the expression and lo-calization of p65 NF-κB protein in liver tissue.Results:Compared with the control group,rats in the model group exhibited marked pathological changes indicative of liver cirrhosis in their hepatic tissue.Serum levels of ALT,AST,TBIL,IL-6,and TNF-α were significantly elevated(P<0.05).Collagen fibre proliferation in the liver tissue was markedly increased,alongside elevated expression of α-SMA,Col1a1,TGF-β1,and p65 NF-κB in the hepatic tissue(P<0.05).Compared with the model group,pathological damage in liver tissue was improved in rats from the positive drug group and the low-and high-dose Hedyotis diffusa groups.Serum levels of ALT,AST,TBIL,IL-6,and TNF-α decreased(P<0.05),collagen fibre proliferation in liver tis-sue diminished,and liver tissue expression of α-SMA,Col1a1,TGF-β1,and p65 NF-κB in liver tissue de-creased(P<0.05).Conclusion:Hedyotis diffusa reduces fibrosis in the liver tissue of rats with cirrhosis by inhibiting inflammation,and the mechanism may be related to the inhibition of NF-κB signaling.
林燕;史国琴;孙园园
甘肃省兰州市第二人民医院药剂科,甘肃 兰州 730000甘肃省兰州市第二人民医院药剂科,甘肃 兰州 730000联勤保障部队第九四〇医院药剂科,甘肃 兰州 730000
白花蛇舌草肝硬化NF-κB纤维化炎症
Hedyotis diffusaLiver cirrhosisNF-κBInflammationFibrosis
《河北医学》 2026 (1)
28-33,6
2024年度兰州市第四批科技计划项目,(编号:2024-4-19)
评论