异莲心碱调节NF-κB/AP-1信号通路对结直肠癌细胞增殖迁移侵袭及凋亡的影响OA

Objective:To investigate the impacts of isoliensinine(ISO)on the proliferation,migration,invasion,and apoptosis of colorectal cancer(CRC)cells by modulating nuclear factor-κB(NF-κB)/activa-tor protein 1(AP-1)signaling pathway.Methods:Caco2 cells were assigned into Control group(normal culture),L-ISO group,M-ISO group,and H-ISO group(added with 10,20,and 40μmol/L ISO,respec-tively);ISO+PMA group[40μmol/L ISO+1μmol/L NF-κB signaling pathway activator phorbol ester(PMA)]randomly.EdU staining was used to measure Caco2 cell proliferation.Scratch assay was applied to measure Caco2 cell migration.Transwell experiment was used to detect Caco2 cell invasion.Flow cytometry was applied to measure apoptosis of Caco2 cells.WB was used to test NF-κB and AP-1 proteins in various groups.Results:The L-ISO,M-ISO,and H-ISO groups had lower EdU positive cell rate,scratch healing rate,invasion number,NF-κB,and AP-1 proteins,and higher apoptosis rate than Control group(P<0.05).The ISO+PMA group had higher EdU positive cell rate,scratch healing rate,invasion number,NF-κB,and AP-1 proteins,and lower apoptosis rate than H-ISO group(P<0.05).Conclusion:ISO can in-hibit the NF-κB/AP-1 signaling pathway,thereby suppressing the proliferation,migration,and invasion of CRC cells and promoting cell apoptosis.