马铃薯品种(系)水溶性维生素含量适应性及稳定性综合分析OA

A total of 26 potato varieties cultivated over two years at three pilot sites were used as materials,and the content of ten water-soluble vitamins in potato tubers(ascorbic acid,thiamine,riboflavin,niacinamide,nicotinic acid,pantothenic acid,pyridoxine,pyridoxamine,biotin,and folic acid)was determined using liquid chromatography-mass spectrometry(LC-MS).Through the combined variance analysis and GGE biplot(genotype+genotype × environment interaction,GGE),the genetic difference of water-soluble vitamin content and environ-mental adaptability and stability in different ecological regions was revealed,and the comprehensive evaluation was carried out by combining the membership function method to screen potato varieties(lines)with excellent perform-ance.The results showed that there were extremely significant differences in the genotype effect,environmental effect,genotype × environment interaction effect,year × environment interaction effect,and genotype × year × environment interaction effect in ascorbic acid,thiamine,riboflavin,nicotinamide,niacin,pyridoxine,and pyridoxamine content,respectively.The GGE biplot analysis indicated that C3 exhibited the highest adaptability in the pilot sites of Weiyuan and Shandan,while H1 performed the highest adaptability in the pilot site of Yongchang.The high and sta-ble vitamin level was identified in C2,H1,and G57 for ascorbic acid;A7,H1,and A4 for thiamine;H2,H7,and G57 for riboflavin;G25,G23,and C5 for nicotinamide;G25,C7,and G23 for nicotinic acid;H2,C4,and C7 for pantothenic acid;G23,H6,and A3 for pyridoxine;H5,C3,and H6 for pyridoxamine;G1,A5,and G25 for biotin;C3,A1,and H3 for folic acid.The discriminative ability of trial sites ranked as follows:Weiyuan in 2022,Yongchang in 2023,Shandan in 2022.Weiyuan had the strongest representativeness both in 2022 and 2023.The inte-gration of the GGE model and the membership function method facilitated a comprehensive evaluation of water-soluble vitamin content in potato cultivars(lines).'H0902'(H1),'Beifang NO.002'(C3),'H0933'(H3),'CIP398180.612'(G23),and'Ivory russet'(A5)showed the best overall performance under three environments.Weiyuan was i-dentified as the ideal site for screening excellent genotypes under multi-environment conditions,while Yongchang and Shandan were more suitable for selecting genotypes with specific water-soluble vitamins.