Fe-N共掺杂碳量子点作为高活性类过氧化物酶用于绿原酸比色检测OA

Fe-N-CDs nanopaticles were successfully prepared by one-step hydrothermal method using L-histidine and Fersulfate heptahydrate(FeSO₄·7H₂O)as precursors.The introduction of Fe atoms produced more active sites on the surface of carbon dots,promoted the electron transfer in the catalytic process,and thus improved the catalytic efficiency of the material.The Fe-N-CDs nanopaticles effectively catalyzed the decomposition of hydrogen peroxide(H2O2)to generate hydroxyl radicals(•OH),which subsequently oxidized 3,3',5,5'-tetramethylbenzidine(TMB)to form a blue oxidation product(oxTMB)with a characteristic absorption peak at 652 nm.Curcumin,a natural polyphe-nolic compound widely found in plants,exhibits excellent antioxidant properties and antidepressant effects.Leveraging the ability of curcumin to reduce oxTMB and cause its color to fade,a quantitative relationship between the change in absorbance and the curcumin content was established.This enabled the development of a colorimetric sensing method for the detection of curcumin.The experimental results demonstrated that this method possesses good selectivity,anti-interference capability and rapid response characteristics.It can be applied for the colorimetric analysis of antioxidant components in pharmaceuticals and food products,offering a new analytical approach for quality assessment of related products and the development of antidepressant drugs.