狗牙根WRKY基因家族鉴定及碱性盐胁迫下表达分析OA

Bermudagrass has important application value in landscaping and ecological restoration because of its strong drought resistance,saline-alkali resistance,and developed rhizomes and stolons.However,the molecu-lar mechanism of its response to alkaline salt stress is still unclear.The WRKY gene family plays an important role in plant growth,development and defense against biotic and abiotic stresses,but it has not been fully identi-fied in bermudagrass.Therefore,based on genomic data,this study systematically identified the physical and chemical properties,chromosome distribution,cis-acting elements,gene structure,conserved motifs and phy-logenetic trees of WRKY gene family members in bermudagrass.At the same time,based on transcriptome data,the expression pattern of its family members under alkaline salt stress was analyzed.The results showed that 129 WRKY transcription factors were identified in the bermudagrass genome and named according to their location on the chromosome.Phylogenetic analysis was carried out by comparing with Arabidopsis WRKY pro-tein,and the above transcription factors were divided into three families:I,II and III,which were unevenly distributed on 18 chromosomes with the largest number of chromosomes 7(22).Collinearity analysis showed that fragment replication mainly drove the evolution of CdWRKYs in bermudagrass.Based on the transcrip-tome data,11 differentially expressed CdWRKYs under alkaline salt stress were screened out.The qRT-PCR analysis showed that the expression levels of CdWRKY59,CdWRKY81,CdWRKY113 and CdWRKY126 reached the peak at 3 h,and their expression folds were 99.6-312.3 times than the control.It is speculated that these WRKY family members might play an important role in alkaline salt stress tolerance.The results of this study analyzed the role of WRKY genes in the response of bermudagrass to alkaline salt stress,and laid a foun-dation for further functional analysis of WRKY gene family.