以凋落物为基质栽培肺形侧耳的配方筛选及营养成分分析OA

The effects of different broad-leaved tree litter on mycelial growth,fruiting body yield and quality of Pleurotus pulmonata were studied by microscopic observation of colony morphology and hyphae,stand-off culture,ITS sequence analysis and plate culture with P.pulmonata strains as test materials,in order to provide reference for expanding the selection range of low-cost cultural substrates and resource utilization of litter of P.pulmonata.The results showed that DL-P.pul001 showed obvious superiority in hyphae growth rate on 7 broad-leaved tree litter substrates,and the growth rate on Tilia amurensis litter was the fastest((4.02±0.19)mm·d-1),significantly faster than that of sawdust control((3.10±0.05)mm·d-1).Furthermore,four formulas were designed to cultivate P.pulmonata DL-P.pul001 with T.amurensis litter as the main substrate.The fruit body yield((133.00±1.55)g·bag-1),stipe diameter((2.24±0.27)cm),capsule length((7.21±0.54)cm),capsule width((6.57±0.36)cm)and crude protein((235.75±0.27)mg·g-1)of P.pulmonata cultivated with formula Ⅲ(55％litter,18％fungus bran,15％wheat bran,10％corncob,1％lime,1％gypsum)were better than those of the sawdust control group.Formula Ⅱ(65％litter,8％fungus chaff,15％wheat bran,10％corncob,1％lime,1％gypsum)was the best in the yield of crude polysaccharide((19.15±0.18)mg·g-1)and crude fat(2.98％±0.09％).