辣椒KNOX基因家族的全基因组鉴定及逆境表达分析OA

Taking cultivated pepper(Capsicum annuum L.)as the test material,bioinformatics analysis was used to study the KNOX gene at the pepper genome level,including the physicochemical properties,gene structure,protein conserved domains,systematic evolution,promoter cis acting elements,and tissue and stress expression patterns of KNOX gene family members,in order to provide reference for the function of KNOX gene in pepper growth and development,as well as stress response.The results showed that there were a total of 7 KNOX gene family members in the pepper genome,distributed on 7 chromosomes.The majority encoded acidic proteins,were all hydrophilic proteins,and were all located in the nucleus.Phylogenetic analysis could divide the pepper KNOX family into two subfamilies,Class Ⅰ and Class Ⅱ,and further into five subgroups.Promoter analysis revealed some cis-acting regulatory elements related to growth and development,hormones,and stress responses.Tissue expression analysis showed that the Class Ⅰ genes had obvious tissue specificity and were mainly highly expressed in roots,stems,and leaves;the Class Ⅱ genes had a more extensive spatiotemporal expression.Under seven abiotic stress treatments,the expression levels of some genes changed significantly,among which five genes responded to specific stresses.RT-qPCR verified two genes that specifically responded to cold stress,salt stress,and heat stress.