Single-cell atlas of grass carp(Ctenopharyngodon idella)peripheral blood IgM^(+)B cells provides insights into B cell-mediated immune responses in teleost fishOACSCDCSTPCD
Teleost peripheral blood contains a remarkably high proportion of B cells,accounting for 15%-50%of circulating lymphocytes.However,their immune responses to bacterial infection are yet to be elucidated.In the present study,10×Genomics single-cell RNA sequencing(scRNA-seq)was employed to characterize the transcriptomic landscape of peripheral blood IgM^(+)B cells in grass carp(Ctenopharyngodon idella)following challenge with Aeromonas hydrophila,a major aquatic pathogen.Six transcriptionally distinct IgM^(+)B cell subpopulations were identified,including(im)mature B cells,innate B cells,proliferating B cells,IgD^(high)B cells,and two infection-induced subsets denoted as infectionⅠandⅡB cells.Bacterial infection altered the cellular heterogeneity of IgM^(+)B cells,triggered metabolic reprogramming in(im)mature and innate B cell subpopulations,and enhanced the immunological activation of circulating B cells.Notably,infectionⅠB cells demonstrated robust induction of interferonφ1(IFNφ1),a type I IFN,following A.hydrophila exposure.This induction was further validated through in vitro bacterial stimulation,indicating that teleost B cells actively contribute to innate antibacterial responses through IFN signaling.Additionally,the IgD^(high)B cell subpopulation remained consistently present in peripheral blood across both infected and uninfected states,pointing to a constitutive and likely mature phenotype.These findings significantly advance our understanding of the heterogeneity of peripheral blood IgM^(+)B cells and provide new insights into IgM^(+)B cell-mediated immune responses in teleost fish.
Yi-Ru Pan;Xue-Qing Han;Tian-Tian Tian;Yong-An Zhang;Xu-Jie Zhang
National Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,ChinaNational Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,ChinaNational Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,ChinaNational Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,China Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Wuhan,Hubei 430070,China Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518124,ChinaNational Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan,Hubei 430070,China Shenzhen Institute of Nutrition and Health,Huazhong Agricultural University,Wuhan,Hubei 430070,China Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518124,China
农业科技
ScRNA-seqB cellsPeripheral bloodGrass carpBacterial infection
《Zoological Research》 2025 (5)
P.953-966,14
supported by the National Natural Science Foundation of China(32373162,31972824)Major Science and Technology Project of Hubei Province(2023BBA001),HZAU-AGIS Cooperation Fund(SZYJY2023006)China Agriculture Research System of MOF and MARA(CARS-46)。
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